Keywords: Spectroscopy, Neuroscience, Chemogenetics, DREADDs, Pharmacology
Motivation: Excitatory designer receptors (DREADDs) may affect glutamatergic and GABAergic neural signaling. Measuring acute neurochemical changes resulting from DREADDs activation using magnetic resonance spectroscopy (MRS) would provide new capabilities for chemogenetic studies.
Goal(s): We investigated the potential for MRS to detect changes to glutamate, GABA, and other neurochemicals following DREADDs activation in the dorsal striatum of mice.
Approach: Mice expressing DREADDs in striatal interneurons completed an MRS session during which DREADDs were activated between two MRS acquisitions.
Results: We detected significant differences in GABA, glutamate, glutamine, choline, and creatine concentrations following activation of the designer receptors. Sex differences were found.
Impact: Our results suggest that magnetic resonance spectroscopy provides noninvasive measurement of changes to glutamate and GABA following acute chemogenetic activation. This presents new opportunities for the optimization of experiments and improved interpretation of results for studies utilizing chemogenetic techniques.
How to access this content:
For one year after publication, abstracts and videos are only open to registrants of this annual meeting. Registrants should use their existing login information. Non-registrant access can be purchased via the ISMRM E-Library.
After one year, current ISMRM & ISMRT members get free access to both the abstracts and videos. Non-members and non-registrants must purchase access via the ISMRM E-Library.
After two years, the meeting proceedings (abstracts) are opened to the public and require no login information. Videos remain behind password for access by members, registrants and E-Library customers.
Keywords