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Abstract #3023

Identifying common metabolic networks altered by glutamine depletion in breast and pancreatic cancer cells

Raj Kumar Sharma1, Noriko Mori1, Balaji Kumar Krishnamachary 1, Yelena Mironchik1, and Zaver M. Bhujwalla1
1The Johns Hopkins University School of Medicine, Baltimore, MD, United States

Synopsis

Keywords: Biomarkers, Metabolism, Cancer metabolism

Motivation: Glutamine plays essential roles in tumor growth and response to treatment.

Goal(s): To understand the impact of Gln depletion on cancer metabolism, we characterized metabolic alterations in pancreatic and breast cancer cells under Gln deprivation .

Approach: We used 1H MRS to understand the metabolic reprogramming caused by glutamine depletion in two pancreatic and two breast cancer cell lines.

Results: The changes in metabolism were accompanied by a significant reduction of cancer cell viability. Glutathione and succinate commonly decreased. We also observed, a significant increase of total choline, glycerophosphocholine and choline that may represent an adaptive metabolic response of cancer cells to glutamine depletion.

Impact: Targeting glutamine metabolism may offer a promising therapeutic approach, as acute glutamine depletion induces significant metabolic reprogramming in PDAC and breast cancer cells.

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