Abstract #3546

MRI Relaxation rates of fecal microbiota from porcine stool samples and their relationship to essential metals

Gabriel VM^1,2, Qin Sun^1,2, Seema Parvathy^3,4, Selinia Hong⁵, Olayori Olasode⁵, Diksha Diksha^1,2,6, Sarah Donnelly¹, Jeremy P. Burton^1,7,8,9, Donna E. Goldhawk^1,2,6, Frank S. Prato^1,2,6,10, Gerald Moran*¹¹, and Neil Gelman*^1,2,10

¹Imaging, Lawson Research Institute, London, ON, Canada, ²Department of Medical Biophysics, Western University, London, ON, Canada, ³Microbiology, Lawson Research Institute, London, ON, Canada, ⁴Division of Infectious Diseases, St. Joseph's Health Care, London, ON, Canada, ⁵Department of Biology, Western University, London, ON, Canada, ⁶Collaborative Graduate Program in Molecular Imaging, Western University, London, ON, Canada, ⁷Department of Microbiology and Immunology, Western University, London, ON, Canada, ⁸Canadian Centre for Human Microbiome and Probiotics Research, London, ON, Canada, ⁹Department of Surgery, Western University, London, ON, Canada, ¹⁰Medical Imaging, Western University, London, ON, Canada, ¹¹Siemens Healthcare Limited, Oakville, ON, Canada

Synopsis

Keywords: Relaxometry, Neurofluids, bacteria imaging, metal content, fecal Microbiota Transplantation (FMT), inductively-coupled plasma mass spectrometry (ICP-MS), contrast enhanced MRI

Motivation: This work is the first step in developing MRI methods to assess and quantify the gut microbiome in humans.

Goal(s): Establish a relationship between MRI relaxation rates and bacterial metal content.

Approach: An FMT procedure was applied to decompose 4 stool samples. Relaxation rates (R₂* (1/T₂*), R₂(1/T₂), R₁(1/T₁) and R₂’=R₂*-R₂) were estimated for each component and the fecal microbiota was further analyzed with a 14-analyte ICP-MS to quantify metal content.

Results: Despite the limited sample size, relaxation rates from fecal microbiota tended to increase with higher concentrations of zinc, iron, and manganese.

Impact: With further validation, this could significantly impact the scope of clinical applications for abdominal MRI towards the detection of gut bacteria given their metal content.

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