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Abstract #3645

Hyperpolarized 13C MRS can detect alterations in the astrocyte-neuron lactate shuttle (ANLS) in the healthy mouse brain

Tamara Vasilkovska1,2, Xiao Gao1,2, Sophie Malaquin2, Laurits Mathiesen2, Marina Radoul2, Jeremy W Gordon1, and Myriam M Chaumeil1,2
1Radiology and Biomedical Imaging, University of California, San Francisco, San Francisco, CA, United States, 2Physical Therapy and Rehabilitation Science, University of California, San Francisco, San Francisco, CA, United States

Synopsis

Keywords: Biology, Models, Methods, Brain, brain energy metabolism

Motivation: To understand how hyperpolarized (HP) 13C MRS can provide information on the astrocyte-neuron lactate shuttle in the healthy brain.

Goal(s): In this study, we assessed changes in whole brain energy metabolism after pharmacological blockage of astrocytic monocarboxylate transporters (MCTs), measured with HP [1-13C]pyruvate MRS.

Approach: Paired HP 13C MRS experiments were performed before and after pharmacological MCT1/4 blocking in 4-month-old C57BL/6J mice, using different combination of metabolite-specific multi-band, spectral spatial (SPSP) sequence.

Results: After astrocytic MCT blockage, the conversion of pyruvate to lactate and bicarbonate was decreased, in line with reduced astrocytic lactate production and neuronal aerobic respiration.

Impact: This study indicates that HP 13C MRS can be used to investigate alterations in the astrocyte-neuron lactate shuttle in the brain, showing the potential in applying this method to investigate metabolic changes in neurological disorders.

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