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Abstract #3866

Receptor-Mediated Labeling of CD4 T cells for Longitudinal Tracking of Distribution Following Systemic Injection in Mouse

Yu Ping1, Songyue Han1, Brock Howerton1, Reuben Adatorwovor2, and Fanny Chapelin1,3,4
1Shu Chien Gene Lay Department of Bioengineering, UCSD, San Diego, CA, United States, 2Biostatistics, University of Kentucky, Lexington, KY, United States, 3Department of Radiology, UCSD, San Diego, CA, United States, 4College of Medicine, University of Kentucky, Lexington, KY, United States

Synopsis

Keywords: Contrast Agents, Contrast Agents, CD4+ T cells, Microbeads, immunomodulation, transplantation

Motivation: This study aims to address the challenge of in vivo tracking of CD4+ T-cells by MRI, focusing on biodistribution monitoring in adoptive cell therapies.

Goal(s): To develop and optimize a receptor-mediated labeling method of CD4+ T-cells with CD4-microbeads that enables in vivo MRI tracking.

Approach: CD4+ T-cells were labeled with CD4-microbeads; internalization and signal attenuation were analyzed via Iron staining, electron microscopy, and MRI. Microbead-labeled cells were injected intravenously, followed by longitudinal MRI tracking and histological analyses.

Results: Internalized CD4-microbeads in T-cells resulted in significant MRI signal reduction, and confirmed the feasibility of tracking labeled T-cells in liver tissue up to 72-hours post-injection.

Impact: The CD4-targeted microbead approach to track CD4+ T cells in vivo with MRI is safe, specific and sensitive. This technique will allow rapid assessment of the efficacy of adoptive cell therapies in conditions such as transplantation and diabetes.

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Keywords