Meeting Banner
Abstract #4752

9.4T MRI-derived myelin measures in post-mortem brain blocks reveal unprecedented spatial characteristics in Multiple Sclerosis lesions

Dimitrios G. Gkotsoulias1,2,3, Ilaria Callegari1,2,3, Jochen Leupold4, Marco Reisert4, Erik Bahn5, Jonas Franz5, Bibek Dhital6, Dominic von Elverfeldt4, Valerij Kiselev4, Matthias Weigel1,2,3, Christine Stadelmann5, and Cristina Granziera1,2,3
1Translational Imaging in Neurology (ThINk), Department of Biomedical Engineering, University of Basel, Basel, Switzerland, 2Research Center for Clinical Neuroimmunology and Neuroscience Basel (RC2NB), University of Basel, Basel, Switzerland, 3Department of Neurology, University of Basel, Basel, Switzerland, 4Division of Medical Physics, Department of Diagnostic and Interventional Radiology, University Medical Center Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany, 5Department of Neuropathology, University Medical Center Göttingen, Göttingen, Germany, 6R.B. Radiology, Kathmandu, Nepal

Synopsis

Keywords: Microstructure, biomarkers

Motivation: Assessing the focal characteristics and extent of microstructural damage and regeneration in MS patients is essential for understanding disease pathogenesis and developing novel therapeutic approaches.

Goal(s): Assessing whether advanced DWI-based measures can provide novel insights into damage and repair in MS lesions.

Approach: Multimodal 9.4T MRI was acquired on lesion-containing post-mortem brain blocks from a patient with MS. The derived metrics include ultra-high-resolution R2*, multishell-DWI-based MD, FA, intra-axonal(Vintra), extra-axonal(Vextra), CSF(VCSF) volume fractions and Myelin-Water-fraction(MWF). Myelin Basic Protein(MBP) immunohistochemistry was employed as myelin ‘ground-truth’.

Results: Vintra revealed spatial inhomogeneities in ROIs within/surrounding MS lesions and stronger association with MBP staining than the corresponding MWF.

Impact: The unprecedented spatial resolution and contrast obtained in post-mortem brain blocks imaged 9.4T MRI open a new window into the assessment of mechanisms leading to focal pathology appearance and evolution in Multiple Sclerosis.

How to access this content:

For one year after publication, abstracts and videos are only open to registrants of this annual meeting. Registrants should use their existing login information. Non-registrant access can be purchased via the ISMRM E-Library.

After one year, current ISMRM & ISMRT members get free access to both the abstracts and videos. Non-members and non-registrants must purchase access via the ISMRM E-Library.

After two years, the meeting proceedings (abstracts) are opened to the public and require no login information. Videos remain behind password for access by members, registrants and E-Library customers.

Click here for more information on becoming a member.

Abstract

Keywords